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靶点科技UbiqBio产品货号Ubiq-054说明书

165 人阅读发布时间:2022-04-03 22:27

产品参数.
Biotin-Ahx-Ub-VME (human sequence, synthetic)
UbiQ code : UbiQ-054 Batch # : B26112012-001
Amount : 50 ug, lyophilized powder
Purity : ≥95% by cation-chromatography and RP-HPLC
Mol. Weight : found 8944 Da, calc 8945 Da
Storage : powder at −20°C, solution at −80°C. Please avoid multiple freeze/thaw cycles.

背景介绍/Background.
Biotin-Ahx-Ub-VME (UbiQ-054) is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs),1 which is labeled on the N-terminus with biotin.2 An aminohexanoic acid linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities

Important: sample preparation
• dissolve the powder in as little DMSO as possible (20 - 40 mg/mL) • add the DMSO stock to milliQ (please note the order of addition) and mix
• buffer the aq. solution as desired (using 1M HEPES or 1M Tris for example) • in general, DMSO concentrations up to 5 vol% are well tolerated by most enzymes.
• If required, total removal of DMSO is accomplished by dialysis or spin-filtration (3 kDa cut-off membrane).
• For detailed experimental conditions please see the open-access reference 1: http://onlinelibrary.wiley.com/doi/10.1002/cbic.201200497/abstract

参考文献/Literature.
(1) Misaghi et al. J. Biol. Chem. 2005, 280, 1512.
(2) de Jong et al. ChemBioChem 2012, 13, 2251.
资料格式:

靶点科技Ubiqbio产品货号UbiQ-054说明书.pdf

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